Publication details

Detekce bakterií způsobujících močové infekce - využití metody Real-Time PCR

Title in English Detection of bacteria causing urinary infections - use Real-Time PCR method


Year of publication 2013
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Diagnostics and treatment of urinary infections is difficult.Urinary infections caused by Mycoplasma genitalium / hominis, Ureaplasma urealyticum / parvum a Chlamydia trachomatis cause the non-specific urethritis. Neisseria gonorrhea is the originator of gonorrhea. All these bacteria are sexually transmitted.The greatest prevalence of sexually transmitted urinary infections is in sexually active men and women aged 15 to 25 years. The basic symptoms of urinary infection include frequent urge to urinate, vaginal discharge or discharges from the urethra, pain and dysuria. Infections are often asymptomatic. Untreated infections can result in serious reproductive malfunction at both genders. The most accurate method of determining is the DNA diagnostics, out of which has recently been used primarily RealTime PCR. The RealTime detection can be performed from the clinical material such as urine, vaginal and cervical smear, sperm or smear from uretra. Our study has analyzed a total of 96 urine samples from sexually active college students. The set of analyzed samples were able to demonstrated the presence of the bacterial originator of Chlamidia trachomatis in just one case. The Ureaplasma urealyticum/parvum has been proved in nine analyzed urine samples and Neisseria gonorrhoea has been detected in one of sample. DNA Mycoplasma genitalium /hominis has not been discovered in the tested samples.

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