Publication details
Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | Acta crystallographica. Section F |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1107/S1744309113002522 |
| Field | Biochemistry |
| Keywords | Mannosidase; Aspergillus niger; Expression; Crystallization |
| Description | beta-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal beta-linked mannoside in various oligomeric saccharide structures. beta-Mannosidase from Asperigillus niger was expressed in Pichia pastoris and purified to clear homogeneity. beta-mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 A resolution. The crystal belonged to space group P1. Monomeric structure was observed in the asymmetric unit. |
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