Publication details

ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

Authors

DING Zhaojun WANG Bangjun MORENO Ignacio DUPLAKOVA Nikoleta SIMON Sibu CARRARO Nicola REEMMER Jesica PENCIK Ales CHEN Xu TEJOS Ricardo SKUPA Petr POLLMANN Stephan MRAVEC Jozef PETRASEK Jan ZAŽÍMALOVÁ Eva HONYS David ROLCIK Jakub MURPHY Angus ORELLANA Ariel GEISLER Markus FRIML Jiří

Year of publication 2012
Type Article in Periodical
Magazine / Source NATURE COMMUNICATIONS
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.ncbi.nlm.nih.gov/pubmed/22760640
Doi http://dx.doi.org/10.1038/ncomms1941
Field Genetics and molecular biology
Keywords PLANT DEVELOPMENT; ENDOPLASMIC-RETICULUM; POLLEN GERMINATION; UDP-GLUCOSE; THALIANA; EFFLUX; BIOSYNTHESIS; EXPRESSION; GRADIENTS; MEMBRANE
Description Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.
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