Publication details

Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis

Authors

KUČERA Jiří BOUCHAL Pavel LOCHMAN Jan POTĚŠIL David JANICZEK Oldřich ZDRÁHAL Zbyněk MANDL Martin

Year of publication 2013
Type Article in Periodical
Magazine / Source Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1007/s10482-012-9872-2
Field Microbiology, virology
Keywords Acidithiobacillus ferrooxidans; elemental sulfur oxidation; ferrous iron oxidation; pyrite oxidation; proteomics; reverse-transcription quantitative PCR
Attached files
Description In contrast to iron-oxidizing Acidithiobacillus ferrooxidans, A. ferrooxidans from a stationary phase elemental sulfur-oxidizing culture exhibited a lag phase in pyrite oxidation, which is similar to its behavior during ferrous iron oxidation. The ability of elemental sulfur-oxidizing A. ferrooxidans to immediately oxidize ferrous iron or pyrite without a lag phase was only observed in bacteria obtained from growing cultures with elemental sulfur. However, these cultures that shifted to ferrous iron oxidation showed a low rate of ferrous iron oxidation while no growth was observed. Two-dimensional gel electrophoresis was used for a quantitative proteomic analysis of the adaptation process when bacteria were switched from elemental sulfur to ferrous iron. A comparison of total cell lysates revealed 39 proteins whose increase or decrease in abundance was related to this phenotypic switching. However, only a few proteins were closely related to iron and sulfur metabolism. Reverse-transcription quantitative PCR was used to further characterize the bacterial adaptation process. The expression profiles of selected genes primarily involved in the ferrous iron oxidation indicated that phenotypic switching is a complex process that includes the activation of genes encoding a membrane protein, maturation proteins, electron transport proteins and their regulators.
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