Publication details

Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17

Authors

TRATSIAK K. DEGTJARIK O. DRIENOVSKA I. CHRÁST Lukáš REZACOVA P. KUTY M. CHALOUPKOVÁ Radka DAMBORSKÝ Jiří KUTA SMATANOVA I.

Type Article in Periodical
Magazine / Source Acta Crystallographica
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1107/S1744309113012979
Field Biochemistry
Keywords Haloalkane Dehalogenases
Description Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 A.
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