Publication details

X ray vs. NMR structure of the N terminal domain of delta subunit of RNA polymerase

Authors

DEMO Gabriel PAPOUŠKOVÁ Veronika KOMÁREK Jan KADEŘÁVEK Pavel OTRUSINOVÁ Olga SRB Pavel RABATINOVA Alzbeta KRASNY Libor ŽÍDEK Lukáš SKLENÁŘ Vladimír WIMMEROVÁ Michaela

Year of publication 2013
Type Conference abstract
MU Faculty or unit

Central European Institute of Technology

Citation
Description The crystal structure of the N terminal domain of delta subunit of RNA polymerase from Bacillus subtilis solved at the resolution of 2.0 A is presented. The X ray structure shows significant differences from the average NMR structure. We systematically investigated the cause of the discrepancies between the NMR and X ray structures, as the pH, presence of metal ions and crystal packing forces.
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