Publication details

Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis.

Authors

BREJCHA Martin STOKLASOVÁ Martina BRYCHTOVÁ Yvona PANOVSKÁ Anna ŠTĚPANOVSKÁ Kristina VAŇKOVÁ Gabriela PLEVOVÁ Karla OLTOVÁ Alexandra HORKÁ Kateřina POSPÍŠILOVÁ Šárka MAYER Jiří DOUBEK Michael

Year of publication 2014
Type Article in Periodical
Magazine / Source Leukemia Research
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://ac.els-cdn.com/S0145212613003743/1-s2.0-S0145212613003743-main.pdf?_tid=94685418-c7f7-11e4-a9b3-00000aacb362&acdnat=1426082868_d96dbfd19537a9a8ff20a6f2dd3b991a
Doi http://dx.doi.org/10.1016/j.leukres.2013.10.019
Field Oncology and hematology
Keywords Chronic lymphocytic leukemia; Clonal evolution; CpG oligonucleotides; Cytogenetics; Fluorescence in situ hybridization; Interleukin-2
Attached files
Description Chronic lymphocytic leukemia (CLL) patients may acquire new chromosome abnormalities during the course of their disease. Clonal evolution (CE) has been detected by conventional chromosome banding (CBA), several groups also confirmed CE with fluorescence in situ hybridization (FISH). At present, there are minimal prospective data on CE frequency determined using a combination of both methods. Therefore, the aim of our study was to prospectively assess CE frequency using a combination of FISH and CBA after stimulation with CpG oligonucleotides and interleukin-2. Between 2008 and 2012, we enrolled 140 patients with previously untreated CLL in a prospective trial evaluating CE using FISH and CBA after stimulation. Patients provided baseline and regular follow-up peripheral blood samples for testing. There was a median of 3 cytogenetic examinations (using both methods) per patient. CE was detected in 15.7% (22/140) of patients using FISH, in 28.6% (40/140) using CBA, and in 34.3% (48/140) of patients by combining both methods. Poor-prognosis CE (new deletion 17p, new deletion 11q or new complex karyotype) was detected in 15% (21/140) of patients and was significantly associated with previous CLL treatment (p=0.013). CBA provides more complex information about cytogenetic abnormalities in CLL patients than FISH and confirms that many patients can acquire new abnormalities during the course of their disease in a relatively short time period.
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