Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling

• Authors: MÉNOVÁ Petra; CAHOVÁ Hana; PLUCNARA Medard; HAVRAN Luděk; FOJTA Miroslav; HOCEK Michal
• Year of publication: 2013
• Type: Article in Periodical
• Magazine / Source: Chemical communications
• MU Faculty or unit: Central European Institute of Technology
• Web: http://www.rsc.org/chemcomm
• Doi: http://dx.doi.org/10.1039/C3CC41438H
• Field: Biochemistry
• Keywords: DNA polymerase; DNA labelling; oligonucleotides
• Description: Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.

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