DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC ACTIVITY

• Authors: TURJAP Miroslav; DOVRTĚLOVÁ Gabriela; NOSKOVÁ Kristýna; ZENDULKA Ondřej; JUŘICA Jan
• Year of publication: 2014
• Type: Article in Proceedings
• Conference: CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS
• MU Faculty or unit: Faculty of Medicine
• Field: Biochemistry
• Keywords: LIQUID-CHROMATOGRAPHY
• Description: The innovative HPLC method for the determination of testosterone and its five metabolites in incubation medium with rat liver microsomes is reported. The method utilizes liquid-liquid extraction procedure, with use of internal standard prednisone. Analytes are eluted within 18.7 min. The method enables reliable determination of testosterone, 7 alpha-hydroxytestosterone, 6 beta-hydroxytestosterone, 16 alpha-hydroxytestosterone, 16 beta-hydroxytestosterone, 2 beta-hydroxytestosterone with LOD in the range 1.9-3.9 nmol/L.

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