Publication details

Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water

Authors

HORKÁ Marie KARÁSEK Pavel RŮŽIČKA Filip DVOŘÁČKOVÁ Milada SITTOVÁ Martina ROTH Michal

Type Article in Periodical
Magazine / Source Analytical Chemistry
MU Faculty or unit

Faculty of Medicine

Citation
Web http://pubs.acs.org/doi/abs/10.1021/ac502254f
Doi http://dx.doi.org/10.1021/ac502254f
Field Analytic chemistry
Keywords FLIGHT MASS-SPECTROMETRY; BACTERIAL ADHESION; ZONE-ELECTROPHORESIS; ISOELECTRIC POINT; CHROMOGENIC AGAR; SPECTRA MRSA; UV DETECTION; MICROORGANISMS; STRAINS; IDENTIFICATION
Description Identification and prevention of Staphylococcus aureus-caused infections may benefit from a fast and dependable method to distinguish between the methicillin-resistant (MRSA) and methicillin susceptible (MSSA) S. aureus strains. The current methods involving polymerase chain reaction and/or other molecular tests are usually laborious and time-consuming. We describe here a fasgt and low-cost method employing capillary zone electrophoresis (CZE) to distinguish between MRSA and MSSA. The method makes use of a supecritical water-treated fused silica capillary, the inner surface of which has subsequently been modified with (3 glycidyloxypropyl)trimethoxysilane. With optimized proportions fo suitable additives to the background electrolyte, a CZE separation of MRSA from MSSA may be completed within 12 min. The cells were baseline-resolved, and resolution was determined to be 3.61. the isoelectric points of MSSA and MRSA were found to be the same for both groups of these strains, pI= 3.4.
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