Publication details

Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.

Authors

AMARO M. BREZOVSKÝ Jan KOVÁČOVÁ Silvia SYKORA J. BEDNÁŘ David NĚMEC Václav LIŠKOVÁ Veronika KURUMBANG Nagendra Prasad BEERENS Koen CHALOUPKOVÁ Radka PARUCH Kamil HOF Martina DAMBORSKÝ Jiří

Year of publication 2015
Type Article in Periodical
Magazine / Source Journal of the American Chemical Society
MU Faculty or unit

Faculty of Science

Citation
Web http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf
Doi http://dx.doi.org/10.1021/jacs.5b01681
Field Biochemistry
Keywords dehalogenases;fluorescence spectroscopy;molecular dynamics simulations
Description Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.
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