Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.
|Year of publication||2015|
|Type||Article in Periodical|
|Magazine / Source||Journal of the American Chemical Society|
|MU Faculty or unit|
|Keywords||dehalogenases;fluorescence spectroscopy;molecular dynamics simulations|
|Description||Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.|