Publication details
An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Periodical |
| Magazine / Source | Frontiers in Plant Science |
| MU Faculty or unit | |
| Citation | |
| Web | http://dx.doi.org/10.3389/fpls.2015.00211 |
| Doi | http://dx.doi.org/10.3389/fpls.2015.00211 |
| Field | Physiology |
| Keywords | conductive elements; dye perfusion; fluorescence; Fluorescent Brightener 28; hydraulic conductivity; vessel; xylem |
| Description | Dye perfusion is commonly used for the identification of conductive elements important for the study of xylem development as well as precise hydraulic estimations. The tiny size of inflorescence stems, the small amount of vessels in close arrangement, and high hydraulic resistivity delimit the use of the method for quantification of the water conductivity of Arabidopsis thaliana, one of the recently most extensively used plant models. Here, we present an extensive adjustment to the method in order to reliably identify individual functional (conductive) vessels. |