Publication details

Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale

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Authors

SEDLMEIER Andreas HLAVÁČEK Antonín BIRNER Lucia MICKERT Matthias MUHR Verena HIRSCH Thomas CORSTJENS Paul TANKE Hans SOUKKA Tero GORRIS Hans

Year of publication 2016
Type Article in Periodical
Magazine / Source Analytical Chemistry
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://pubs.acs.org/doi/full/10.1021/acs.analchem.5b04147
Doi http://dx.doi.org/10.1021/acs.analchem.5b04147
Field Analytic chemistry
Keywords CONVERTING PHOSPHOR REPORTERS; LANTHANIDE-DOPED NANOCRYSTALS; UP-CONVERSION NANOPARTICLES; CIRCULATING ANODIC ANTIGEN; LATERAL FLOW ASSAY; IMAGING IN-VIVO; FLUORESCENT; CELLS; NANOPHOSPHORS; LUMINESCENCE
Description An upconversion laser scanner has been optimized to exploit the advantages of photon-upconverting nanoparticles (UCNPs) for background-free imaging on a macroscopic scale. A collimated 980 nm laser beam afforded high local excitation densities to account for the nonlinear luminescence response of UCNPs. As few as 2000 nano particles were detectable, and the linear dynamic range covered more than 5 orders of magnitude, which is essentially impossible by using conventional fluorescent dyes. UCNPs covered by a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventional fluorescence scanner as well as the upconversion scanner. Both optical labels could be detected independently. Finally, upconversion images of lateral flow test strips were recorded to facilitate the sensitive and quantitative detection of disease markers. A marker for the parasitic worm Schistosoma was used in this study.
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