Publication details

Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae)



Year of publication 2016
Type Article in Periodical
Magazine / Source Czech Journal of Animal Science
MU Faculty or unit

Faculty of Science

Field Microbiology, virology
Keywords gut microflora; selective media; identification; Enterobacter; Aeromonas
Description In this study, the intestinal bacteria of wild Antarctic fish (family Nototeniidae) were examined with traditional culture-based techniques. The bacterial flora of the gut contents of four marine fish species Notothenia coriiceps, Trematomus bernacchii, Trematomus hansoni and Trematomus newnesi has been investigated in an attempt to describe the allochthonous bacteria inhabiting these Nototheniidae fish. A set of 43 fresh and healthy fish was analysed and intestinal bacteria were retrieved using the dilution plate technique on selective media Endo agar and XLDA agar. A total of 133 different bacterial isolates were obtained and initially characterised by key phenotypical tests. Notothenoid fish gut microbiota showed low species diversity of isolates and intestines were inhabited in average amount of 3 different isolates per fish. The poor bacterial colonization of intestine content showed samples of Trematomus newnesi. Curiously, the Gram-negative non-fermenters, including Pseudomonas sp., Vibrio sp. and Alcaligenes-like isolates represented the majority (59%) of intestine isolates grown on the used selective media for enteric bacteria. Based on preliminary identification, only 54 isolates (41%) were tentatively determined as enteric bacteria. The identification of 54 isolates of Gram-negative fermenting rods to the species level was achieved using biochemical characterization by commercial kits ENTEROtest 24 and Biolog GN2 MicroPlate. Results showed that Enterobacter cloacae phenon and Aeromonas hydrophila were predominant bacterial species in the free-living fish intestine from the group of fermenting Gram-negative rods.
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