Publication details

Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology.

Authors

ŠKORPÍKOVÁ Lucie GELNAR Milan SLANÝ Michal KAŠNÝ Martin KRÁLÍK Petr ILGOVÁ Jana SNÍŽKOVÁ Karolína

Year of publication 2016
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Science

Citation
Description Roundworms of the genus Trichinella are clinically important parasites invading cells of the small intestine and skeletal muscles. They infect a broad range of hosts involving birds, reptiles and mammals. Seven of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious human disease. During the life cycle, these parasites release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. They are essential for many biological processes like migration, digestion, development, and also in manipulation of the host immunne system. The dominant components of the E-S products are cystatins - reversible inhibitors of cysteine peptidases of C1 (papain-like) family and rarely of C13 (legumain-like) family. All the representatives of cystatin superfamily are evolutionary related and share some sequential and structural identity. Our work is focused on the characterization of "multi cystatin-like domain" (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared in Escherichia coli expression system. Currently, the recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnostics of Trichinella. We employed the xMAP technology - novel diagnostic platform based on magnetic microspheres and a signal evaluation by MAGPIX device.
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