Publication details

BACE enzyme kinetics detected by MALDI TOF mass spectrometry

Title in English BACE ENZYME KINETICS DETECTED BY MALDI TOF MASS SPECTROMETRY
Authors

MACHÁLKOVÁ Markéta SCHEJBAL Jan GLATZ Zdeněk PREISLER Jan

Year of publication 2016
Type Article in Proceedings
Conference CECE 2016
MU Faculty or unit

Faculty of Science

Citation
Web http://www.ce-ce.org/CECE2016/Proceedings%20CECE%202016_WOS.pdf
Field Analytic chemistry
Keywords kinetics; enzyme; MALDI; BACE; mass spectrometry; inhibition
Description Matrix-assisted laser desorption/ionization mass spectrometry was introduced for the analysis of enzyme kinetics and inhibition. The Km for selected substrate and IC50 values for enzyme inhibitor were in a good agreement with results obtained by capillary electrophoresis coupled to electrospray mass spectrometry. More specifically, it was focused on beta secretase (BACE), an enzyme playing important role in Alzheimer’s disease development. Its selective inhibition in brain could be beneficial for the patients; thus, specific inhibitor of this enzyme was examined as well. In comparison with traditional fluorometric or colorimetric methods, the both mass spectrometric approaches offered faster, sensitive and more precise BACE analysis.
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