Publication details

Electrophoretically mediated microanalysis for on-capillary derivatization of amino acids using naphthalene-2,3-dicarboxaldehyde and sodium cyanide


CELÁ Andrea MÁDR Aleš ŠULCOVÁ Martina GLATZ Zdeněk

Year of publication 2016
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Amino acids are very important compounds for living organisms and their determination can reveal physiological state of an organism. They exhibit low or no native fluorescence, thus derivatization is essential for their sensitive determination by laser induced fluorescence detection. Derivatization reaction can be done in pre-capillary, on capillary or post capillary arrangement. On capillary derivatization provides many advantages, such as requirement of smaller volumes of sample and other reagents and possibility of automation. Mixing of the reactants in narrow capillaries can be performed by longitudinal diffusion, by transverse diffusion of laminar flow profiles (TDLFP), or by electrophoretically mediated microanalysis (EMMA). Optimization of EMMA for derivatization of all standard AAs using naphthalene-2,3-dicarboxaldehyde and sodium cyanide and comparison with derivatization of AAs using TDLFP methodology are subjects of this contribution.
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