Publication details

Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL

Authors

MRÁZKOVÁ Jana MALINOVSKÁ Lenka WIMMEROVÁ Michaela

Year of publication 2017
Type Chapter of a book
MU Faculty or unit

Faculty of Science

Citation
Description In this chapter, we describe a site-directed mutagenesis method used for investigating the sugar binding pattern of the PA-IIL lectin from the pathogenic bacterium Pseudomonas aeruginosa . Moreover, procedures for the production and purification of PA-IIL mutants are described, and several basic methods for characterizing the mutants are discussed.
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