Publication details

Clusters of Monoisotopic Elements for Calibration in (TOF) Mass Spectrometry

Authors

KOLÁŘOVÁ Lenka PROKEŠ Lubomír KUČERA Lukáš HAMPL Aleš PEŇA MENDÉZ Eladia Maria VAŇHARA Petr HAVEL Josef

Year of publication 2017
Type Article in Periodical
Magazine / Source Journal of The American Society for Mass Spectrometry
MU Faculty or unit

Faculty of Science

Citation
Web https://link.springer.com/article/10.1007%2Fs13361-016-1567-x
Doi http://dx.doi.org/10.1007/s13361-016-1567-x
Field Analytic chemistry
Keywords Monoisotopic elements; Calibration; Gold clusters; Phosphorus clusters; TOF mass spectrometry
Description Precise calibration in TOF MS requires suitable and reliable standards, which are not always available for high masses. We evaluated inorganic clusters of the monoisotopic elements gold and phosphorus (Aun+/Aun- and Pn+/Pn-) as an alternative to peptides or proteins for the external and internal calibration of mass spectra in various experimental and instrumental scenarios. Monoisotopic gold or phosphorus clusters can be easily generated in situ from suitable precursors by laser desorption/ionization (LDI) or matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Their use offers numerous advantages, including simplicity of preparation, biological inertness, and exact mass determination even at lower mass resolution. We used citrate-stabilized gold nanoparticles to generate gold calibration clusters, and red phosphorus powder to generate phosphorus clusters. Both elements can be added to samples to perform internal calibration up to mass-to-charge (m/z) 10–15,000 without significantly interfering with the analyte. We demonstrated the use of the gold and phosphorous clusters in the MS analysis of complex biological samples, including microbial standards and total extracts of mouse embryonic fibroblasts. We believe that clusters of monoisotopic elements could be used as generally applicable calibrants for complex biological samples.
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