Publication details

Role of IFN-α in multifactorial respiratory disease of swine



Year of publication 2017
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Porcine reproductive and respiratory syndrome virus (PRRSV) can predispose pigs to the secondary respiratory infection with bacteria such as Haemophilus parasuis. Animals infected with both pathogens develop more severe clinical disease. Macrophages serve as the target of replication of PRRSV. There are two types of macrophages – porcine alveolar macrophages (PAMs) as resident cells provide one of the first lines of defence against microbes invading the lung tissue. On the other hand, monocyte-derived macrophages (MDMs) as inflammatory macrophages are naive cells accumulating in site of inflammation. Concurrent infection of PAMs or MDMs with PRRSV and/or H. parasuis was analysed in vitro and difference dependent on macrophage type were observed. MDMs were more sensitive to PRRSV infection resulting in higher mortality of cells and higher production of IFN-? compared to fully differentiated PAMs. Elevated level of IFN-? decreased expression of pro-inflammatory cytokines (IL-1ß and IL-8) what was confirmed also by experimental addition of IFN-? to MDMs followed by infection with H. parasuis. In addition, MDMs infected with H. parasuis alone, but not in co-infection, formed multinucleated giant cells (MGCs). Infection with PRRSV could therefore facilitate the development of a secondary bacterial infection by avoiding the formation of MGCs by macrophages. Higher sensitivity of undifferentiated macrophages accumulating in site of inflammation could contribute to the development of multifactorial respiratory disease of swine. The work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (project LO1218) and the Ministry of Agriculture of the Czech Republic (QJ1210120).

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