Publication details

Tolerance of isolated rabbit hearts to short ischemic periods is affected by increased LV mass fraction

Authors

HLAVÁČOVÁ Miroslava OLEJNÍČKOVÁ Veronika RONZHINA M. STRAČINA Tibor JANOUŠEK O. NOVÁKOVÁ Marie BABULA Petr KOLÁŘOVÁ J. PROVAZNÍK I. PAULOVÁ Hana

Year of publication 2017
Type Article in Periodical
Magazine / Source Physiological research
MU Faculty or unit

Faculty of Medicine

Citation
Field Biochemistry
Keywords Rabbit isolated heart; LV mass; Global ischemia; Electrogram; 4-hydroxy-2-nonenal
Description Hypertrophied hearts are known for increased risk of arrhythmias and are linked with reduced ischemic tolerance. However, still little is known about state characterized only by increased left ventricle (LV) mass fraction. Seventeen isolated rabbit hearts with various LV mass were divided into two groups according to LV weight/heart weight ratio (LVW/HW ratio), namely group H and L (with higher and lower LVW/HW ratio, respectively) and underwent three short cycles of global ischemia and reperfusion. The differences in electrogram (heart rate, QRSmax, mean number, onset and dominant form of ventricular premature beats) and in biochemical markers of myocardial injury (creatine kinase, lactate dehydrogenase – LDH) and lipid peroxidation (4-hydroxy-2-nonenal – 4-HNE) were studied. As compared to group L, hearts in group H exhibited lower tolerance to ischemia expressed as higher incidence and severity of arrhythmias in the first ischemic period as well as increase of LDH and 4-HNE after the first reperfusion. In the third cycle of ischemia-reperfusion, the preconditioning effect was observed in both electrophysiological parameters and LDH release in group H. Our results showed consistent trends when comparing changes in electrograms and biochemical markers. Moreover, 4-HNE seems to be good potential parameter of moderate membrane alteration following ischemia-reperfusion injury.
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