Publication details

New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria



Year of publication 2017
Type Article in Proceedings
Conference MendelNet 2017 Proceedings of 24th International PhD Students Conference
MU Faculty or unit

Faculty of Science

Field Microbiology, virology
Keywords sulfate-reducing bacteria; Desulfovibrio; cultivation media; modification of conditions; bacterial growth
Description Different genera of sulfate-reducing bacteria (SRB) are always detected in the large intestine of humans and animals with diseases like an ulcerative colitis and Crohn's disease or even cancer. The final metabolism product of these anaerobic microorganisms is hydrogen sulfide which is known as a toxic substance and can lead to damage of epithelial cells of the bowel in high concentration. Some genera of the intestinal SRB included to the Desulfovibrionaceae family are hard to cultivate or even uncultivable. Isolation of these genera is also complicated because there are others satellite microorganisms. Up to now, Postgate’s medium and other media do still not solve the cultivation problem and are created generally for Desulfovibrio species from nature environment but not for SRB species from the intestine. The object of our research was to modify the principle of isolation of intestinal SRB and cultivation medium based on their physiological and biochemical properties. Thus, there is no selective medium for intestine SRB which would improve cultivation and isolation of these important microorganisms. New created medium can be useful for more opportunities of intestinal SRB cultivation and understanding their involvement in inflammatory bowel diseases.
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