Publication details

The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report

Authors

LIPOVÝ Břetislav MATEJOVIČOVÁ Milena ŘIHOVÁ H. ŠTIKAROVSKÁ M. NOVOTNÁ Lucie HLAVÁČOVÁ Miroslava PAULOVÁ Hana SUCHÁNEK I. BRYCHTA Pavel

Year of publication 2017
Type Article in Periodical
Magazine / Source Burns Open
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1016/j.burnso.2017.05.007
Field Surgery incl. transplantology
Keywords Toxic epidermal necrolysis; Immunosuppression; Comet assay
Description Toxic epidermal necrolysis (TEN) represents the most serious affection within the SCARs group (Severe Cutaneous Adverse Reactions). It is an autoimmune disease manifested by extensive epidermal and mucosal exfoliation. The basic approach in the therapy of patients suffering from toxic epidermal necrolysis is the administration of immunosuppressive medications. Comet assay is a fast, sensitive and relatively easy method used to detect DNA damage levels. We present a case report of a woman with toxic epidermal necrolysis treated with cyclosporine A. During the therapy, comet assay was used to detect the level of DNA damage of circulating lymphocytes and it helped us to reveal qualitative abnormalities in these cells.