Publication details

Biosensorická špička s LinA a LinB pro detekci beta-HCH

Title in English The biosensor tip with LinA and LinB for detection beta-HCH
Authors

MOSKALÍKOVÁ Hana KOTLÁNOVÁ Markéta PROKOP Zbyněk ŘEZÁČ Jaroslav

MU Faculty or unit

Faculty of Science

Description The newly developed prototype of biosensor tip sensitive to beta-HCH combines two initial enzymes of the metabolic pathway for degradation of lindane, dehydrochlorinase LinA and haloalkane dehalogenase LinB. Both enzymes have been stabilized by protein engineering and exhibit increased stability during immobilization techniques. A new biosensor tip utilize the high density of two co-immobilized proteins and exhibit sensitivity in the detection of beta-1,2,3,4,5,6-hexachlorocyclohexane (a problematic environmental contaminant included in the Stockholm Convention).
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