Publication details

Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells

Authors

BOSTROM Johan ŠRÁMKOVÁ Zuzana SALAŠOVÁ Alena JOHARD Helena Anna Dagmar MAHDESSIAN Diana FEDR Radek MARKS Carolyn MEDALOVÁ Jiřina SOUČEK Karel LUNDBERG Emma LINNARSON Sten BRYJA Vítězslav SEKYROVÁ Petra ALTUN Mikael ANDÄNG Michael

Year of publication 2017
Type Article in Periodical
Magazine / Source PLOS ONE
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724894/pdf/pone.0188772.pdf
Doi http://dx.doi.org/10.1371/journal.pone.0188772
Field Genetics and molecular biology
Keywords cell cycle; synchronization; fucci reporter
Description The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchronized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.
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