Publication details

Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material

Authors

GRILLOVÁ Linda GIACANI Lorenzo PAŠTĚKOVÁ Lenka STROUHAL Michal STRNADEL Radim MARRA Christina CENTURION-LARA Arturo POVEDA Lucy RUSSO Giancarlo ČEJKOVÁ Darina VAŠKŮ Vladimír OPPELT Jan ŠMAJS David

Year of publication 2018
Type Article in Periodical
Magazine / Source Plos one
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1371/journal.pone.0202619
Keywords Treponema pallidum
Description Treponema pallidum subsp. pallidum (TPA) is the infectious agent of syphilis, a disease that infects more than 5 million people annually. Since TPA is an uncultivable bacterium, most of the information on TPA genetics comes from genome sequencing and molecular typing studies. This study presents the first complete TPA genome (without sequencing gaps) of clinical isolate (UZ1974), which was obtained directly from clinical material, without multiplication in rabbits. Whole genome sequencing was performed using a newly developed Anti-Treponemal Antibody Enrichment technique combined with previously reported Pooled Segment Genome Sequencing. We identified the UW074B genome, isolated from a sample previously propagated in rabbits, to be the closest relative of the UZ1974 genome and calculated the TPA mutation rate as 2.8 x 10(-10) per site per generation.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info