Publication details

Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

Authors

TVRDOŇOVÁ Michaela VLČNOVSKÁ Marcela VANICKOVA Lucie KANICKÝ Viktor ADAM Vojtěch ASCHER Lena JAKUBOWSKI Norbert VACULOVIČOVÁ Markéta VACULOVIČ Tomáš

Year of publication 2019
Type Article in Periodical
Magazine / Source Analytical and Bioanalytical chemistry
MU Faculty or unit

Faculty of Science

Citation
Web http://dx.doi.org/10.1007/s00216-018-1300-7
Doi http://dx.doi.org/10.1007/s00216-018-1300-7
Keywords Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry
Description In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
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