Publication details

Capillary electrophoretic analysis of ionic content in exhaled breath condensate and pH monitoring as a non-invasive method in gastroesophageal reflux disease diagnostics

Authors

LAČNÁ Júlia ĎURČ Pavol GREGUŠ Michal SKŘIČKOVÁ Jana DOUBKOVÁ Martina POKOJOVÁ Eva KINDLOVÁ Dagmar DOLINA Jiří KONEČNÝ Štefan FORET František KUBÁŇ Petr

Year of publication 2019
Type Article in Periodical
Magazine / Source Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://www.sciencedirect.com/science/article/pii/S1570023219313297?via%3Dihub
Doi http://dx.doi.org/10.1016/j.jchromb.2019.121857
Keywords Capillary electrophoresis; Contactless conductivity detection; Exhaled breath condensate; Anions and cations; pH; Gastroesophageal reflux disease
Description In this study, the ionic profile and pH of exhaled breath condensate (EBC) in a group of patients with acid and weakly acid reflux and no-reflux controls were compared. A portable sampler was used for non-invasive EBC collection from five exhalations. The ionic profile (anions, cations, organic acids) and pH of the collected EBC samples were measured by capillary electrophoresis with contactless conductivity detection and a pH microelectrode, respectively. Several ions were elevated in the patient groups. Sodium cation was elevated in weakly acid reflux (significance level p < 0.01) and acid reflux (p < 0.05) compared to no-reflux controls. Butyrate and propionate were elevated in both acid reflux and weakly acid reflux compared to no-reflux controls (butyrate: p < 0.01, propionate: p < 0.05). The median values of pH (after de-aeration with N2) were also significantly higher (p < 0.01) in groups with acid reflux and weakly acid reflux than in the control group with no reflux. The ionic analysis and simultaneous pH measurement offer a simple, cheap, fast, and non-invasive approach in gastroesophageal reflux disease diagnostics. © 2019 Elsevier B.V.
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