Publication details

Automated workflow for the study of drug-protein interactions based on capillary electrophoresis-frontal analysis combined with in-capillary mixing of interacting partners

Authors

GLATZ Zdeněk MICHALCOVÁ Lenka NEVÍDALOVÁ Hana

Year of publication 2019
Type Conference abstract
Citation
Description In this study, the new CE-FA method was created. The in-line approach of the sample mixing directly inside the capillary is based on diffusion and it is generic, robust and rapid. The numerous injection parameters and incubation period were considered and optimized. The method was used to determine the binding parameters of model systems (propranolol/lidocaine BSA) and compared with the classic methods. Based on the statistical evaluation, it was found that the results achieved both approaches are almost identical. In-line sample mixing for the CE FA method has so far not been published and a combination of CE FA approaches gives rise to a fully automated and versatile method for characterizing binding interactions.
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