Publication details

MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer

Authors

VYCHYTILOVÁ Petra SVOBODOVÁ KOVAŘÍKOVÁ Alena GROLICH Tomáš PROCHÁZKA Vladimír SLABÁ Kateřina MACHÁČKOVÁ Táňa HALÁMKOVÁ Jana SVOBODA Marek KALA Zdeněk KISS Igor SLABÝ Ondřej

Year of publication 2019
Type Article in Periodical
Magazine / Source International Journal of Molecular Sciences
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://www.mdpi.com/1422-0067/20/18/4460
Doi http://dx.doi.org/10.3390/ijms20184460
Keywords microRNA; biogenesis; colorectal cancer; disease-free survival; overall survival; RT-qPCR
Description MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. Each step of their production and maturation has to be strictly regulated, as any disruption of control mechanisms may lead to cancer. Thus, we have measured the expression of 19 genes involved in miRNAs biogenesis pathway in tumor tissues of 239 colorectal cancer (CRC) patients, 17 CRC patients with liver metastases and 239 adjacent tissues using real-time PCR. Subsequently, the expression of analyzed genes was correlated with the clinical-pathological features as well as with the survival of patients. In total, significant over-expression of all analyzed genes was observed in tumor tissues as well as in liver metastases except for LIN28A/B. Furthermore, it was shown that the deregulated levels of some of the analyzed genes significantly correlate with tumor stage, grade, location, size and lymph node positivity. Finally, high levels of DROSHA and TARBP2 were associated with shorter disease-free survival, while the over-expression of XPO5, TNRC6A and DDX17 was detected in tissues of patients with shorter overall survival and poor prognosis. Our data indicate that changed levels of miRNA biogenesis genes may contribute to origin as well as progression of CRC; thus, these molecules could serve as potential therapeutic targets.
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