Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene

• Authors: STARA Jitka; PEKÁR Stanislav; NESVORNA Marta; ERBAN Tomas; VINSOVA Hana; KOPECKY Jan; DOSKOCIL Ivo; KAMLER Martin; HUBERT Jan
• Year of publication: 2019
• Type: Article in Periodical
• Magazine / Source: Experimental and Applied Acarology
• MU Faculty or unit: Faculty of Science
• web: https://link.springer.com/article/10.1007%2Fs10493-019-00353-9
• Doi: https://doi.org/10.1007/s10493-019-00353-9
• Keywords: Varroa destructor; Honey bee; Apiary; Acaricide; Restriction
• Description: In this study, we used the discriminating concentration of tau-fluvalinate (0.25 mu g/mL) to detect the resistance of mites in a bioassay. Further, we verified the presence of the kdr mutation in mites from the bioassay via PCR amplification of a fragment of the voltage-gated sodium channel gene (Na(V)Ch), restriction fragment length polymorphisms (RFLPs), and densitometry analyses in pools of surviving or dead mites.