Publication details

Generation of human iPSCs from fetal prostate fibroblasts HPrF

Authors

KAHOUNOVA Zuzana SLABAKOVA Eva BINO Lucia REMSIK Jan FEDR Radek BOUCHAL Jan VRTEL Radek JUREČKOVÁ Lucie POROKH Volodymyr PARALOVA Darja HAMPL Aleš SOUCEK Karel

Year of publication 2019
Type Article in Periodical
Magazine / Source Stem cell research
MU Faculty or unit

Faculty of Medicine

Citation
Web https://www.sciencedirect.com/science/article/pii/S1873506119300352
Doi http://dx.doi.org/10.1016/j.scr.2019.101405
Keywords iPSCs; fetal prostate fibroblasts
Description Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune (TM)-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.
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