Publication details

The use of high resolution melting analysis of ITS-1 for rapid differentiation of parasitic nematodes Haemonchus contortus and Ashworthius sidemi

Authors

ŠKORPÍKOVÁ Lucie RESLOVÁ Nikol MAGDÁLEK Jan VADLEJCH Jaroslav KAŠNÝ Martin

Year of publication 2020
Type Article in Periodical
Magazine / Source Scientific reports
MU Faculty or unit

Faculty of Science

Citation
Web https://www.nature.com/articles/s41598-020-73037-9
Doi http://dx.doi.org/10.1038/s41598-020-73037-9
Keywords gastrointestinal nematodes; ruminants; haemonchus contortus; ashworthius sidemi; species differentiation
Attached files
Description Among gastrointestinal nematodes, haematophagous strongylids Haemonchus contortus and Ashworthius sidemi belong to the most pathogenic parasites of both domestic and wild ruminants. Correct identification of parasitic taxa is of crucial importance in many areas of parasite research, including monitoring of occurrence, epidemiological studies, or testing of effectiveness of therapy. In this study, we identified H. contortus and A. sidemi in a broad range of ruminant hosts that occur in the Czech Republic using morphological/morphometric and molecular approaches. As an advanced molecular method, we employed qPCR followed by High Resolution Melting analysis, specifically targeting the internal transcribed spacer 1 (ITS-1) sequence to distinguish the two nematode species. We demonstrate that High Resolution Melting curves allow for taxonomic affiliation, making it a convenient, rapid, and reliable identification tool.
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