Publication details

LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis

Authors

VLČNOVSKÁ Marcela KUCHYNKA Michaela ŠTOSSOVÁ Aneta HOLCOVÁ POLANSKÁ Hana VACULOVICOVA Marketa VACULOVIČ Tomáš MASAŘÍK Michal

Year of publication 2019
Type Article in Proceedings
Conference MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE
MU Faculty or unit

Faculty of Science

Citation
Web https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4
Keywords gold nanoparticles; LA-ICP-MS; immunochemistry; dot-blot; protein detection; p53
Description Laser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step.
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