Publication details

Implication of TRPC3 channel in gustatory perception of dietary lipids

Authors

MURTAZA Babar HICHAMI Aziz KHAN Amira S. PLESNÍK Jiří ŠERÝ Omar DIETRICH Alexander BIRNBAUMER Lutz KHAN Naim A.

Year of publication 2021
Type Article in Periodical
Magazine / Source Acta Physiologica
MU Faculty or unit

Faculty of Science

Citation
Web https://doi.org/10.1111/apha.13554
Doi http://dx.doi.org/10.1111/apha.13554
Keywords Ca(2+)signalling; fat taste; fatty acids; lipids; TRPC3 channels
Description Aim The pathogenesis of obesity has been associated with high intake of dietary fat, and some recent studies have explored the cellular mechanisms of oro-sensory detection of dietary fatty acids. We further assessed the role of transient receptor potential canonical (TRPC) channels in oro-sensory perception of dietary lipids. Methods We determined by RT-qPCR and western blotting the expression of TRPC3/6/7 channels in mouse fungiform taste bud cells (mTBC). Immunocytochemistry was used to explore whether TRPC3 channels were co-expressed with fatty acid receptors. We employed wild-type (WT) mTBC, and those transfected with small interfering RNAs (siRNAs) against TRPC3 or STIM1. Ca(2+)signalling was studied in TBC fromTRPC3(-/-)mice and their WT littermates. Results We demonstrate that mouse fungiform taste bud cells (mTBC) express TRPC3, but not TRPC6 or TRPC7 channels, and their inactivation by siRNA or experiments on TBC fromTRPC3(-/-)mice brought about a decrease in fatty acid-induced gustatory Ca(2+)signalling, coupled with taste bud CD36 lipid sensor. TRPC3 channel activation was found to be under the control of STIM1 in lingual mTBC. Behavioural studies showed that spontaneous preference for a dietary long-chain fatty acid was abolished inTRPC3(-/-)mice, and in mice wherein lingual TRPC3 expression was silenced by employing siRNA. Conclusion We report that lingual TRPC3 channels are critically involved in fat taste perception.

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