Publication details

Upconversion nanoparticles as labels for histopathological tissue evaluation

Authors

MICKERT Matthias Jürgen FARKA Zdeněk KRAWCZYK Krzysztof HLAVÁČEK Antonín BRANDMEIER Julian ANDERSSON-ENGELS Stefan SJÖGREN Anders SKLÁDAL Petr GORRIS Hans-Heiner

Year of publication 2021
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Immunocytochemistry (ICC) and immunohistochemistry (IHC) are routine methods for the microscopic identification of cancerous cells in cell cultures and histological tissues. A growing population and longer life expectancy lead to an increase in tissue samples from cancer patients. Multiplexing and digital pathology can increase the sample throughput, improve the diagnosis, and reduce the analysis time. UCNPs are well suited for digital image analysis of biological tissues because the anti-Stokes emission under NIR excitation drastically reduces autofluorescence and light scattering, and their multiple narrow emission bands allow for multiplexed applications. In this work, we designed streptavidin-modified UCNPs (SA-UCNP) for the detection of the breast cancer biomarker HER2 on the surface of model breast cancer cell lines and breast cancer tissue. The SA-UCNP conjugate achieved a significantly higher signal-to-background ratio compared to a conventional labelling using a streptavidin-carboxyfluorescein conjugate under the same experimental conditions. An in-house made UCNP-conjugate from Lumito was used to label HER2 in breast cancer tissue. The imaging was performed by Lumito’s prototype scanner capable of combining UCNP upconversion imaging and haematoxylin brightfield imaging at a 20× magnification.
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