Publication details
DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome
| Authors | |
|---|---|
| Year of publication | 2021 |
| Type | Article in Periodical |
| Magazine / Source | eLife |
| MU Faculty or unit | |
| Citation | |
| Web | https://elifesciences.org/articles/60916#xb0ffa41b |
| Doi | http://dx.doi.org/10.7554/eLife.60916 |
| Keywords | PORTAL-VEIN; ARTERIOHEPATIC DYSPLASIA; HEPATIC-ARTERY; HUMAN JAGGED1; LIVER; MUTATIONS; INSIGHTS; NETWORK; ORGAN |
| Description | Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1(Ndr/Ndr) mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1(Ndr/Ndr) liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models. |