Publication details

Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

Authors

BRANDMEIER Julian RAIKO Kirsti FARKA Zdeněk PELTOMAA Riikka MICKERT Matthias Jürgen HLAVÁČEK Antonín SKLÁDAL Petr SOUKKA Tero GORRIS Hans-Heiner

Year of publication 2021
Type Article in Periodical
Magazine / Source Advanced Healthcare Materials
MU Faculty or unit

Faculty of Science

Citation
Web https://onlinelibrary.wiley.com/doi/10.1002/adhm.202100506
Doi http://dx.doi.org/10.1002/adhm.202100506
Keywords anti-Stokes emission; cardiac arrest; lanthadine-dopoed nanomaterials; single-molecule immunoassay; troponin; photon-upconversion; upconversion-linked immunosorbent assay
Description Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL-1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.
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