Publication details

Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic



Year of publication 2021
Type Article in Periodical
Magazine / Source Life
MU Faculty or unit

Faculty of Medicine

Web text článku
Keywords beta-lactamse, antiibotic resistance, PCR, Acinetobacter baumannii, bacteria
Description Increasing antimicrobial resistance in nosocomial pathogens, such as Acinetobacter bauman- nii, is becoming a serious threat to public health. It is necessary to detect ß-lactamase-producing microorganisms in clinical settings to be able to control the spread of carbapenem resistance. This study was conducted to evaluate the presence of ß-lactamases in a selected clinical isolate of A. baumannii of ST2P/ST195Ox and to characterize possible enzymes, as well as its ß-lactam resistome, using PCR and whole-genome sequencing analysis. PCR and sequencing con?rmed that the isolate harbored ?ve bla gene alleles, namely, blaADC-73, blaTEM-1, blaOXA-23, blaOXA-58 and blaOXA-66, as well as aminoglycosides, macrolides, sulfonamides and tetracyclines resistance determinants, which were either chromosomally and/or plasmid located. Furthermore, a gene order comparison using MAUVE alignment showed multiple changes compared with the clinical isolate of Malaysian A. baumannii AC30 genome and 76 regions with high homology. This study suggests that resistance to ß-lactams in this A. baumannii isolate is mainly due to an overproduction of ß-lactamases in combination with other resistance mechanism (ef?ux pump system).

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