Publication details

Komparace bakteriomu z tumoru a ze sliznic dutiny ústní u pacientů s orálním dlaždicobuněčným karcinomem

Title in English Comparison of bacterioma from tumor and oral mucosa in patients with oral squamous cell carcinoma


Year of publication 2022
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Introduction: Oral squamous cell carcinoma (OSCC) is the most common malignancy in the head and neck region. The presence of Gram-negative anaerobes, such as Fusobacterium nucleatum and Treponema denticola, in the oral cavity is associated with this disease. The oral microbiome is known to be location-dependent, e.g. the tongue dorsum and buccal mucosa are dominated by Gram-positive Steptococcus. The aim of this pilot study is to analyze the oral bacteriom in different locations of the oral cavity in patients with OSCC and to determine whether the bacteriom on the tumor surface differs from that from locations without clinical findings. Methods: Three oral mucosal swabs (tumor surface, buccal mucosa, and tongue without clinical findings) were collected from 33 patients with histopathologically confirmed diagnosis of OSCC. Microbial DNA was isolated from each matrix using the QIAamp DNA Mini Kit (QIAGEN), and the 16S rRNA hypervariable region was amplified. Subsequently, the library was prepared for next-generation sequencing on an Illumina MiSeq instrument. Results: The most abundant genera in the oral samples were Streptococcus, Fusobacterium, Prevotella_7 and Veillonella. The alpha diversity of the bacteriome was comparable between matrices (p > 0.05). However, the relative abundance for the genus Rothia was significantly lower in the tumor swab compared to the buccal mucosa or tongue (p ? 0.001, q = 0.003). The genera Actinomyces and Atopobium were less abundant on the tumor surface, whereas Prevotella_2, Parvimonas, Catonella, Peptostreptococcus, Fusobacterium, Aggregatibacter and Treponema were more abundant when compared against the tongue surface (q < 0.05) but not against buccal swab (p > 0.05). The genus Streptococcus was found to have a lower relative abundance in the tumor swab than in the buccal mucosa (q < 0.05). Summary: In patients with OSCC, the bacteriome of the tumor surface is different from that of the tongue or buccal mucosa. The higher prevalence of periodontal bacteria on the tumor surface compared to other mucosal sites without obvious pathology supports the hypothesis of their importance in the etiopathogenesis of this disease. Pilot results need to be verified in a larger sample of patients.
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