Publication details

Nature of Low density neutrophils in patients with Common variable immunodeficiency



Year of publication 2022
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Description Common variable immunodeficiency disorder (CVID) is the most common form of clinically significant primary immunodeficiency. Majority of patients receive intravenous immunoglobulin (IVIg) replacement therapy with potent immunomodulatory properties on individual components of the immune system. CVID is associated with a chronic granulocytic activation and an increased percentage of Low Density Neutrophils (LDN) observed in the layer of peripheral blood mononuclear cells (PBMC). The aim of the project was to determine properties of LDN in patients with CVID, compare them with the neutrophils in the peripheral blood and observe the possible effect of IVIg administration. Changes in the percentage of LDN in PBMC and expression of their surface markers were studied in 25 CVID patients and 27 healthy donors (HD) in a whole blood samples and after in-vitro stimulation of whole blood with IVIg. Samples of whole blood and PBMC were stimulated with E.coli for determination of neutrophil respiratory burst. We confirmed an increased number of LDN in the PBMC layer in a fresh blood samples from CVID patients. PBMC samples of CVID patients consisted of 0,20 % immature and 0,74 % mature LDN when compared with 0,11% immature and 0,24% mature LDN of HD group distinguished based on the expression of CD10 and CD16. The division of neutrophils into immature and mature population was confirmed by confocal fluorescence microscopy. The flow cytometry analysis revealed decreased expression of CD10 on mature LDN of CVID patients. We observed that mature LDNs of CVID patients have significantly reduced respiratory burst compared to mature LDNs of HD, whereas there was no difference in respiratory burst of mature neutrophils in the whole blood between both experimental groups. Stimulation of whole blood with IVIg caused an increased number of LDN in both CVID patients and HD. The addition of IVIg inhibited the expression of CD11b on the mature LDN in both experimental groups. We conclude that mature LDNs of CVID patients have incomplete differentiation that may be related to a reduced ability of respiratory burst. Our results also show that IVIg supports the formation of LDN, which can affect the immune response of CVID patients.
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