Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods

• Authors: VANÍČKOVÁ Lucie; DO Tomáš; VEJVODOVÁ Markéta; HORÁK Vratislav; HUBÁLEK Martin; EMRI Gabriella; ZEMÁNKOVÁ Kristýna; PAVELICOVÁ Kristýna; KŘÍŽKOVÁ Soňa; FALTUSOVÁ Veronika; POMPEIANO Antonio; VACULOVIČOVÁ Markéta; ZÍTKA Ondřej; VACULOVIČ Tomáš; ADAM Vojtěch
• Year of publication: 2022
• Type: Article in Periodical
• Magazine / Source: International Journal of Biological Macromolecules
• MU Faculty or unit: Faculty of Science
• web: https://doi.org/10.1016/j.ijbiomac.2022.01.139
• Doi: https://doi.org/10.1016/j.ijbiomac.2022.01.139
• Keywords: Melanoma; Mass spectrometry; MeLiM
• Description: Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins.