Publication details

Transkriptomický profil interakce terapeutického bakteriofága z rodu Kayvirus s bakterií Staphylococcus aureus

Title in English Transcriptomic profile of the interaction of a Kayvirus therapeutic bacteriophage with Staphylococcus aureus


Year of publication 2022
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description The treatment of human and animal diseases caused by pathogenic bacteria Staphylococcus aureus is becoming increasingly complicated due to increasing antimicrobial resistance. An alternative to antibiotics currently used to treat staphylococcal infections is phage treatment by bacteriophages from the genus Kayvirus. Still, their wider use is limited by the need for more information on the interaction between the phage and its host at the molecular level. In the presented study, two strains of S. aureus were infected with kayvirus bacteriophage K. Changes in phage and bacterial gene expression during infection were monitored using RNA sequencing. The transcriptional profile during the phage life cycle was comparable in both strains, except for a few genes encoding hypothetical proteins. Data from RNA sequencing also indicated the presence of phage non-coding RNAs that could influence the expression of both phage and bacterial genes. The host response to phage infection resembled the general bacterial stress response at the transcriptomic level. In both strains of S. aureus, the expression of genes for nucleotide synthesis, amino acid synthesis, transport proteins and energy metabolism increased. At the same time, a decrease in the expression of genes for three bacterial transcription factors was observed. The obtained results clarify the phage-host interaction at the transcriptional level, significantly contribute to the safer use of phages in therapy, and reveal the possibilities of developing new strategies for eradicating bacterial pathogens.
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