Publication details
Image processing and analysis of light sheet microscopy data
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| Year of publication | 2024 |
| Type | Chapter of a book |
| Citation | |
| Description | Image processing of light sheet data involves several specific challenges that are not encountered in other imaging modalities. The ability to image the same specimen from multiple angles necessitates image registration and fusion before the data can be used for subsequent analysis. The solution to this preprocessing task is complicated by the large volume of the image data acquired with any light sheet microscope. The scale of the data also affects the downstream analysis of objects of biological interest in the imagery. While the tasks are similar to other imaging modalities –segmentation, tracking, and atlas registration – the volume of the data requires the development of dedicated solutions on both the software and hardware sides. This chapter provides an overview of the available solutions to tackle the preprocessing and analysis of light sheet data at scale using primarily open-source image analysis platforms. |