Publication details
Exploring the impacts of CRISPR/Cas-mediated chromosome rearrangements on plant genome dynamics
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| Year of publication | 2024 |
| Type | Conference abstract |
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| Description | The global climate continuously challenges the plant genetic engineering industry to provide novel plant varieties with an unprecedented level of resistance without sacrificing yield. For this purpose, CRISPR/Cas-mediated large-scale chromosome rearrangements, imitating those that occurred naturally during plant evolution, can be utilized. These rearrangements often have significant implications for the plant's genetic makeup and epigenetic regulation. While most current studies focus on the process of constructing such plants, our research looks one step ahead as we study future generations of modified plants to uncover whether and how these chromosome re-arrangements will be reflected. To this end, we have carried out analyses of plant phenotype, telomere lengths, transcript levels, and structural changes within chromatin using subsequent generations of Arabidopsis thaliana plants with stable reciprocal translocations of the ends of long arms of chromosomes 1-2 and 1-5 (Beying et al., 2020; doi: 10.1038/s41477-020-0663-x). Telomeres were analyzed in A. thaliana lines with inverted chromosome 2 (Rönspies et al., 2022; doi: 10.1038/s41477-022-01238-3). The outcomes of our research will not only reveal the impact of the chromosome rearrangements on the organization of the genome but also the interconnection of these changes. Understanding the impact of chromosomal rearrangements in Arabidopsis thaliana can provide valuable insights into the evolutionary dynamics and adaptive potential of plant genomes. Funding: Czech Science Foundation, 22-04364S P 013 |
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