Publication details
SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS
| Authors | |
|---|---|
| Year of publication | 2024 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Sample preparation is crucial for oligosaccharide and glycan analysis by chromatographic or electrophoretic methods. Since native oligosaccharides and glycans lack chromophores or fluorophores, they require molecular modification for optical detection. Adding a charged group also enhances electrophoretic mobility and ionization efficiency for MS detection. Standard oligosaccharides and N-linked glycans were derivatized with newly synthesized fluorescent and MS-active labels. After purification, the labeled compounds were separated by CE or LC and detected using fluorescence or MS. Novel labels based on 2-phenylpyridine derivatives were created, allowing tuning of fluorescence and MS properties. Compared to commercially available labels, these new labels showed higher reaction yields, tunable fluorescence, and better ionization efficiency. This work showcases the development of novel fluorescent labels with fast reaction kinetics, high fluorescence yields, and improved MS detectability. |
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