Publication details
Influence of Rat Bodyweight During the Testing of Antiarrhythmic Compounds Against Ventricular Tachyarrhythmias on Langendorff Hearts
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| Year of publication | 2025 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Variations in bodyweight may impact myocardial structure, coronary perfusion, and pharmacodynamic responses, potentially altering the heart's susceptibility to arrhythmias and its responsiveness to therapeutic agents. In this study, we investigated the effect of rat bodyweight on the testing of potential antiarrhythmic compounds by using our regional ischemia model on Langendorff apparatus. Hearts from 15 male Wistar albino rats were divided into two groups based on bodyweight: Group A (400–540 g, n=8) and Group B (134–196 g, n=7). Each group was further subdivided into control and testing subgroups (Group A: control n=4, testing n=4; Group B: control n=3, testing n=4). Reperfusion-induced ventricular tachyarrhythmias (VTA) occurred with 100% incidence in both groups undergoing 10 minutes of regional ischemia through occlusion of the left main artery, followed by 15 minutes of reperfusion after removing the occlusion. Testing group hearts received the infusion of potential antiarrhythmic compound BF-Tpip-HCl during the first 10 minutes of reperfusion while the control group hearts received only 40% PEG solution. Test compound was dissolved in 5 ml 40% PEG-distilled water solution. During the experiments, heart rate (bpm), coronary flow (ml/min) and VTA duration (sec) in all hearts were collected and analysed. Results showed that differences of heart rate (mean±SD) between Group A (908±292) and Group B (411±53) in reperfusion period were statistically significant (p<0.05). Moreover, VTA duration in Group A (465±299) was significantly higher than Group B (26±28) in reperfusion (p<0.05). |
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