Publication details
hIR-TEV-TwinStrep and hIRecto-TEV-TwinStrep plasmids for expression of human insulin receptor and its insulin binding ectodomain in insect cells
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Outcomes put into operation (prototype, working sample) |
| MU Faculty or unit | |
| Attached files | |
| Description | The functional sample consists of donor plasmids enabling expression of full-length human insulin receptor or its extracellular insulin-binding domain in insect cells using the Bac-to-Bac baculovirus system. The constructs include a C-terminal TEV-cleavable Twin-Strep tag and are optimized for preparative purification and downstream structural studies. |
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