Publication details
New fluorescent markers for glycans analysis
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Changes in the composition of glycans correlate with the progression of many diseases, so they are studied as disease markers. However, their analysis is difficult because of the absence of sufficient chromophores. Therefore, they must be labeled with fluorescent molecules. Our goal was to develop a series of new fluorescent markers covalently bound to glycans providing fast labeling kinetics and high quantum yields, allowing glycans to be studied chromatographically (HPLC). The fluorofor series consists of five molecules differing by substitution on a phenyl fragment (Me, MeO, F). These substitutions will provide molecules with different physicochemical properties. The fluorescent skeleton is based on pyrroindolizine structure. Each fluorophore molecule is prepared in two variants: with a side chain capable of neutral glycan labeling (hydrazide) and aminoglycan labeling (N-hydroxysuccinimide ester). The prepared structures will be characterized in cooperation with the Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic. The usability of the newly developed fluorophores will be demonstrated by profiling glycoproteins associated with breast cancer. |
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