Publication details
Výroba vícevrstvé buněčné náhrady kůže na bázi biokompatibilních pěnových nosičů a kultivovaných autologních/allogenních MSC a keratinocytů
| Title in English | Production of a multilayer cellular skin substitute based on biocompatible foam scaffolds and cultured autologous/allogeneic MSCs and keratinocytes |
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| Authors | |
| Year of publication | 2025 |
| Type | Pilot plant, certified technology, variety, breed |
| MU Faculty or unit | |
| Description | The verified technology concerns the production of functional multilayer skin cell replacements intended for regenerative medicine. The development of this type of replacement represents a fundamental step forward in the field of tissue engineering, as it addresses the shortcomings of current treatments for extensive burns and chronic non-healing wounds, in particular the problem of limited donor sites and the poor quality of newly formed skin coverings. The skin is a complex multilayered organ consisting of the epidermis, dermis, and hypodermis, whose cell populations are arranged in a three-dimensional extracellular matrix. Cell-free biomaterials alone are unable to fully restore both the anatomical and functional structure of the skin. Therefore, the technology uses biocompatible foam carriers based on natural biopolymers, whose physicochemical properties mimic the native extracellular matrix, and combines them with mesenchymal stromal cells (MSCs). The integration of MSCs in a defined type of differentiation into skin fibroblasts and preadipocytes allows the creation of multilayer constructs that model the functional specialization of the individual dermis and hypodermis and provide mechanical and biological parameters close to native tissue. Cultured donor keratinocytes are able to build a fully functional epidermis in this model. The technology enables the creation of functional samples with defined mechanical and biological properties suitable for skin regeneration and is designed for further development towards full-thickness skin replacement with key physiological functions such as the barrier role of the epidermis, the viscoelasticity of the dermis, and the ability to integrate into host tissue. The robustness, feasibility, and reproducibility of the entire production process were evaluated during verification. Biological functionality was confirmed by a series of in vitro tests including cell viability, absence of cytotoxic effects of the carrier, and the ability of differentiated MSCs to maintain their function after integration into a multilayer structure. The functionality of the construct was subsequently verified in vivo in an animal model, where the stability, biological activity, and suitability of the technology for clinical applications were demonstrated. The technology was verified at the Center for Cell and Tissue Engineering at St. Anne's University Hospital in Brno and implemented by the team of Doc. RNDr. Irena Koutná, Ph.D. The results of the verification process, carried out in accordance with GMP principles, confirm the applicability of the technology and provide a solid basis for its standardization, introduction into GMP production, and compilation of clinical use. |
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